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This descriptive, cross-sectional study conducted in Ibadan, South-West Nigeria, explored sexual abstinence practices of in-school adolescents, factors influencing or obstructing abstinence, and knowledge of HIV and AIDS. A multi-stage sampling technique was used to identify 420 respondents who completed a semi-structured questionnaire and participated in focus groups. Eight focus groups were held, with nine participants on average. Qualitative data were transcribed and manually summarized, while the quantitative data was analyzed using the Statistical Package for the Social Sciences. …