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The scope of this protocol encompasses the legal definition of discrimination as well as the identification and elimination of arbitrary discrimination -as defined by a range of important international instruments. Designed to assist National AIDS Programmes and others in detecting arbitrary discrimination, the protocol also serves as an effective human rights tool and a means of enforcing measures against such discrimination. Sources of arbitrary discrimination, and the criteria for assessing their existence, are outlined together with a detailed inductive methodology. The protocol also includes questionnaires for the assessment of arbitrary discrimination and for direct witness/key informant interviews, as well as checklists and orientation programmes for investigators.